Catalase And Coagulase Test Pdf

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Catalase Test- Principle, Uses, Procedure, Result Interpretation with Precautions

The enzyme coagulase , produced by a few of the Staphylococcus species, is a key feature of pathogenic Staph. The enzyme produces coagulation of blood, allowing the organism to "wall " its infection off from the host's protective mechanisms rather effectively. There are 2 methods: slide test and tube test. The tube test is more accurate, but the slide test is faster.

If you have a Staph unknown, you might consider running the slide test, and, if negative, running the tube test. The tube method is THE definitive test. Pathogenic Staph species e. Staph aureus can be confirmed using the coagulase test. Since there are 2 kinds of coagulase enzymes—bound and freethere are 2 different tests that can be used to identify these enzymes. Both of the enzymes activate fibrinogen in plasma, in different ways. Your instructor will direct you as to which procedure to use, but the tube test is the definitive test : if you get a negative test result for the slide test, run the tube test.

Be sure that you have a Staph before running this test: the plasma is expensive. It is a waste to run the test on a non- Staph isolate. Objectives Identify the presence of the enzyme coagulase. Note Be sure that you have a Staph before running this test: the plasma is expensive. Place two drops of thawed rabbit plasma into the circle, using a wooden pick or a clean loop. Fibrin threads form between the cells, causing them to agglutinate, or clump.

There will a visible clumping of cells within seconds. This test is for the bound coagulase enzyme. Any degree of coagulation is considered a positive test for the free coagulase enzyme.

The presence of the coagulase enzyme is an indicator of:.

31: Coagulase Test

The enzyme coagulase , produced by a few of the Staphylococcus species, is a key feature of pathogenic Staph. The enzyme produces coagulation of blood, allowing the organism to "wall " its infection off from the host's protective mechanisms rather effectively. There are 2 methods: slide test and tube test. The tube test is more accurate, but the slide test is faster. If you have a Staph unknown, you might consider running the slide test, and, if negative, running the tube test.


Enzyme-based tests play a crucial part in the identification of bacteria. In. , a publication by Gottstein brought attention to bacterial catalase.


31: Coagulase Test

Metrics details. The ideal identification of Staphylococcus aureus clinical isolates requires a battery of tests and this is costly in resource limited settings. In many developing countries, the tube coagulase test is usually confirmatory for S. This study evaluated Mannitol salt agar and the deoxyribonuclease DNase test for improving the efficiency of the tube coagulase test in resource limited settings. The efficiency of human and sheep plasma with tube coagulase tests was also evaluated.

NCBI Bookshelf. Baron S, editor. Medical Microbiology. Staphylococci can cause many forms of infection.

I) Catalase Test

U.S. Food and Drug Administration

This test demonstrate the presence of catalase, an enzyme that catalyses the release of oxygen from hydrogen peroxide H 2 O 2. It is used to differentiate those bacteria that produces an enzyme catalase, such as staphylococci , from non-catalase producing bacteria such as streptococci. The enzyme catalase mediates the breakdown of hydrogen peroxide into oxygen and water. The presence of the enzyme in a bacterial isolate is evident when a small inoculum is introduced into hydrogen peroxide, and the rapid elaboration of oxygen bubbles occurs. The lack of catalase is evident by a lack of or weak bubble production. The culture should not be more than 24 hours old. Bacteria thereby protect themselves from the lethal effect of Hydrogen peroxide which is accumulated as an end product of aerobic carbohydrate metabolism.

Blood agar is a rich, non-selective medium that supports the growth of most bacteria. However, it is differential based on the ability of the organism to produce enzymes called hemolysins, which lyse red blood cells RBC. Three patterns of hemolysis can be observed on a blood agar plate. Beta b -hemolysis: complete lysis of RBC - A clear zone with a clean edge around the colony.

Purpose or Uses of Catalase Test

Reagents should not be used if there are signs of deterioration or if the expiration date has passed. The test can be done by two methods. This method measures bound coagulase. The bound coagulase is also known as clumping factor. As a result, individual coccus stick to each other and clumping is observed.

We report a catalase-negative Staphylococcus aureus isolated from a year-old male diabetic patient with foot ulcer who attended our surgery ward. Species identification was confirmed by Gram staining, standard biochemical tests and PCR amplification of the nuc and fem genes. Antibiotic susceptibility showed that the strain was sensitive to imepenem, chloramphenicol, amoxicillin, vancomycin and resistant to oxacillin, penicillin, ceftriaxone, erythromycin, clindamycin, and amikacin. Clinicians and microbiologists must be encouraged to identify and report these atypical strains and the infections associated with them in order to establish their role in pathogenesis. Staphylococcus aureus is a gram positive, catalase and coagulase positive coccus and by far the most important pathogen among the staphylococci.

Bennett ret. Lancette ret. Staphylococcus aureus is highly vulnerable to destruction by heat treatment and nearly all sanitizing agents. Thus, the presence of this bacterium or its enterotoxins in processed foods or on food processing equipment is generally an indication of poor sanitation. It has been identified as the causative agent in many food poisoning outbreaks and is probably responsible for even more cases in individuals and family groups than the records show. Foods are examined for the presence of S. Conclusions regarding the significance of S.

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4 Comments

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  2. Г‰dith P. 09.05.2021 at 04:48

    I) Catalase Test. Materials Required: Cultures: hour tryptic soy broth cultures of bacteria. Media: Tryptic soy agar. Reagent: 3% hydrogen peroxide.

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  4. Peregrino M. 12.05.2021 at 02:11

    Bubbling indicated a positive catalase test and that the bacteria were staph bacteria. The bacteria were also tested for coagulase. Both slide and tube coagulase.